Part:BBa_K2246007:Experience

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Applications of BBa_K2246007

Sequencing For the following samples of BSLA devices (Blue chromoprotein, forward siRNA, loop and reverse siRNA): BSLA-AWD, BSLA-SOD, BSLA-WNT and BSLA-RACI the sequencing was made using the VF2 primer, although it was possible to confirm the presence of the blue chromoprotein, the reaction couldn´t show us the presence of the siRNAs constructs because of the length of the protein gene before them. A repetition of the sequencing using the VR primers for the BSLA-SOD and BSLA-RACI devices was performed to seek the siRNA construction before the blue chromoprotein, however the results weren´t conclusive since the alignment showed the correct base pairs just after the reverse primer but not the same sequence before the terminator.

>Sequence obtained with the forward primer (VF2) AGGGATATTCCAGCAATTTTGCCCGTGCCGTCGAAAGGCCCACCCGTGAAGGTGAGCCAGTGAGTTGATTGCTACGTAATTAGTTAGTTAGCCCTTAGTGACTCGAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAATACAAGCTACTTGTTCTTTTTGCATACTAGAGAAAGAGGAGAAATACTAGATGAGTGTGATCGCTAAACAAATGACCTACAAGGTTTATATGTCAGGCACGGTCAATGGACACTACTTTGAGGTCGAAGGCGATGGAAAAGGTAAGCCCTACGAGGGGGAGCAGACGGTAAAGCTCACTGTCACCAAGGGCGGACCTCTGCCATTTGCTTGGGATATTTTATCACCACAGTGTCAGTACGGAAGCATACCATTCACCAAGTACCCTGAAGACATCCCTGACTATGTAAAGCAGTCATTCCCGGAGGGCTATACATGGGAGAGGATCATGAACTTTGAAGATGGTGCAGTGTGTACTGTCAGCAATGATTCCAGCATCCAAGCAACTGTTTCATCTACCATGTCAAGTTCTCTGGTTTTGAACTTTCCTCCCAATGGACCTGGTCTTGCAGAAAGAAAGAACAAAGGGCTGGGAACCCCAC

User Reviews

UNIQ51bb655f9a0d9927-partinfo-00000000-QINU UNIQ51bb655f9a0d9927-partinfo-00000001-QINU